KN-595 EGET
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Patent No.7495680※
Patent No.7704344※
Inventor
・Osaka Metropolitan University (Formerly of Iwate Univ.), Professor Taketo Kaneko
・Institute for Animal Reproduction
EGET stands for
Easy get next Generation by Embryo Transfer
Conventionally, in order to “produce offspring from transplanted fertilized eggs”,
it was required to create pseudopregnant females by vasectomized males.
“Pseudopregnant mouse and rat production device ~EGET~”
does not require a vasectomized male, achieves artificial pseudopregnancy induction.
This method enables the production of spawn from transplanted fertilized eggs in a compact,
and simple operation. It is expected to reduce the number of animals used and the time required.
In addition, pseudopregnant animals can be produced on the day of transplantation.
It can also be used as a backup for conventional methods.
| KN-595-35/40 | KN-595-50 | |
| Target Animal | Mouse | Rat |
| Dimention of Insertion Part |
φ3.5mm×L27mm×1ea (Blue) φ4.0mm×L27mm×1ea (Green) |
φ5.0mm×L27mm×2ea(White) |
| Power | Battery-powered (Battery is not replaceable) | |
| One year of use or used for 100 mice ※Assumed number of uses in case of 30sec x 7 times stimulation |
One year of use or used 250 rats ※Assumed number of uses in case of 30sec x 3 times stimulation |
|
| *Due to the functionality of the product, please use it within the time limit stated above. | ||
*Please comply with local and institutional rules of disposal, or send the entire box of products to us for proper disposal.
*Each EGET is designed to be used 750 times for 30 seconds. The standard stimulation frequency is 30sec x 7 times for mice and 30sec x 3 times for rats.
*The number of times of stimulation is expected to be shortened when the technique is stabilized, so please inquire separately.
*Manufactured and sold by Natsume Seisakusho Co., Ltd. under a patent license agreement.
• Set contents: 1 main unit, 2 tip probes, Instruction manual
＊More detailed information on how to use the product
is available on the buyers-only page.
■ Information Related to EGET (Reference Literature Included)
*The paper summaries in this section have been machine-translated. Please refer to the original articles below for complete and accurate details.
①Intravaginal Progesterone for Estrous Cycle Synchronization
A new method that transforms mouse breeding. Simply applying a progesterone cream intravaginally synchronizes the estrous cycle with high precision, and combining it with an anti-inhibin antibody increases litter size to 1.6× that of natural mating. Pseudopregnant females can also be produced on schedule without vasectomized males—low-invasive and labor-saving, achieving both reduced animal numbers and greater experimental efficiency.
Sato B, Yamashita Y, Shindo M, et al.
Intravaginal Progesterone Application as an Efficient and Reproducible Tool for Synchronizing the Mouse Estrous Cycle.
Reprod Med Biol. 2026;25(1):e70027. doi:10.1002/rmb2.70027
②Application of Easy-ET to Mice
We extended our sonic-vibration-based pseudopregnancy induction method (Easy-ET), originally developed in rats, to mice, successfully generating genome-edited mice as well. By eliminating the need for vasectomized males, this approach reduces housing space and costs while enabling pseudopregnancy induction, genome editing, and embryo transfer to be completed within a single day.
Wake Y, Endo M, Tsunoda S, et al.
Successful induction of pseudopregnancy using sonic vibration in mice.
Sci Rep. 2023;13(1):3604. Published 2023 Mar 3. doi:10.1038/s41598-023-30774-x
③Shortened Stimulation Protocol for Easy-ET
Pseudopregnancy induction is an essential step for embryo transfer. This study shows that even when the sonic-vibration stimulation of the Easy-ET method is shortened from 7×30 s to just 2×30 s, pseudopregnancy is still successfully induced and normal offspring are obtained. It also enables a same-day protocol—stimulation and embryo transfer performed together on the day of estrus—streamlining workflows such as genome-edited animal production.
Endo M, Tsunoda S, Tawara H, et al.
Successful pseudopregnancy of rats by short period artificial stimulation using sonic vibration.
Sci Rep. 2022;12(1):1187. Published 2022 Jan 24. doi:10.1038/s41598-022-05293-w
④Establishment of Easy-ET Using Sonic Vibration
Easy-ET: a novel method that induces pseudopregnancy in rats using only sonic vibration stimulation—no mating with vasectomised males required—and successfully yields offspring via embryo transfer. By eliminating the need to maintain vasectomised males, it cuts housing space and cost while streamlining strain production and maintenance, including for genome-edited rat lines. A must-know technique for researchers.
Kaneko T, Endo M, Tsunoda S, et al.
Simple induction of pseudopregnancy by artificial stimulation using a sonic vibration in rats.
Sci Rep. 2020;10(1):2729. Published 2020 Feb 17. doi:10.1038/s41598-020-59611-1
【Media and Other Publication Information】
https://www.nazme.co.jp/images/2023/10/LABIO21_No.92_p.20-22.pdf
2024.05.01 LABIO21 No.92 (p.23–26) Natsume Seisakusho (Japanese)
https://www.nazme.co.jp/images/2023/10/LABIO21_No.92_p.23-26.pdf
2023.10.30 Iwate Univ. (Japanese)
https://www.iwate-u.ac.jp/cat-research/2023/10/005929.html
2023.05.26 Science Japan
https://sj.jst.go.jp/news/202305/n0526-02k.html
2023.03.06 Press Release (Japanese)
https://www.iwate-u.ac.jp/upload/a4c7844421e0e1688b6f3a296b34f044.pdf
If you have relevant knowledge, data, or literature related to this topic, we would greatly appreciate your contribution. With the aim of improving experimental reproducibility and promoting animal welfare, we may review and consider sharing such information on this page.
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